Obesity & Adiposity
NNMT inhibition with 5-Amino-1MQ reduced white adipose tissue mass by ~35% in diet-induced obese mouse models without suppressing food intake, suggesting energy expenditure modulation.
Hong S et al. 2015≥99% Purity
5-Amino-1MQ
NNMT Inhibition Studies
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About this compound
5-Amino-1MQ is a small molecule that blocks NNMT, an enzyme tied to fat storage. It's widely researched for reducing fat mass, revving up metabolism, and boosting cellular energy (NAD+).
Small-molecule NNMT inhibitor (5-amino-1-methylquinolinium)
- Formula
- C10H10N2
- Molecular weight
- 158.19 g/mol
- Form
- Lyophilized powder
- CAS / ID
- 4213-45-0
0%
Fat Mass Reduction
White adipose tissue in DIO mouse models
0.0×
NAD+ Elevation
Intracellular NAD+ increase post-inhibition
0+
Published Studies
NNMT inhibition research papers
0
Research Areas
Obesity & Adiposity · NAD+ Salvage Pathway · SAM Methyl Donor Pool & more
How It Works
How 5-Amino-1MQ works
The pathways 5-Amino-1MQ acts on — and what each one does. The animation traces its signal outward from the compound to every target it engages.
Signalling pathways studied in preclinical models · illustrative
Nicotinamide N-Methyltransferase Blockade
NNMT Inhibition
5-Amino-1MQ is a small-molecule competitive inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes the methylation of nicotinamide using S-adenosyl methionine (SAM). NNMT is overexpressed in adipose tissue of obese subjects, and its inhibition has been explored as a strategy to modulate methyl group flux and NAD+ precursor availability.
- Competitive inhibitor of NNMT enzyme activity
- Reduces 1-methylnicotinamide (MNA) production
- Preserves SAM for other methylation reactions
NAD+ Precursor Salvage Pathway Modulation
NAD+ Metabolism
By blocking NNMT-mediated consumption of nicotinamide, 5-Amino-1MQ redirects nicotinamide back into the NAD+ salvage pathway. Preclinical studies demonstrate elevated intracellular NAD+ levels following NNMT inhibition, suggesting a role in metabolic energy regulation.
- Redirects nicotinamide into NAD+ salvage pathway
- Elevates intracellular NAD+ (~1.6× in preclinical models)
- Modulates NAMPT-dependent NAD+ biosynthesis
Adipose Tissue Energy Expenditure
Adipocyte Metabolism
In diet-induced obese (DIO) mouse models, NNMT inhibition with 5-Amino-1MQ was associated with reduced white adipose tissue mass without affecting food intake, suggesting changes in energy expenditure rather than appetite suppression. Researchers observed upregulation of metabolic gene expression in adipocytes.
- Reduced white adipose tissue mass in DIO mouse models
- No change in food intake observed
- Altered adipocyte gene expression patterns
Uses & Applications
What 5-Amino-1MQ is researched for
The main areas 5-Amino-1MQ is being studied for — and the study-reported figures behind them.
NAD+ Salvage Pathway
5-Amino-1MQ preserves nicotinamide for NAD+ biosynthesis by blocking NNMT-catalyzed methylation, elevating intracellular NAD+ in adipocyte cell lines.
Kannt A et al. 2015SAM Methyl Donor Pool
NNMT consumes SAM as a methyl donor, competing with DNA and histone methyltransferases. NNMT inhibition may influence epigenetic programming in metabolic tissues.
Pissios P. 2017Glucose Homeostasis
NNMT knockdown in adipocytes was associated with improved markers of insulin sensitivity and glucose uptake in preclinical in vitro models, linking NNMT to metabolic homeostasis.
Kraus D et al. 2014Molecular Structure
The 5-Amino-1MQ molecule
An interactive 3D model rendered from the compound record — rotate and explore its structure.
C10H10N2
Third-Party Verified
Independently tested. Verifiably pure.
Every batch of 5-Amino-1MQ is sent to an accredited independent laboratory before it ships. Here is what we screen for.
- HPLC Purity AnalysisConfirms the peptide is ≥99% pure
- Mass SpectrometryVerifies the exact molecular identity
- Heavy Metals ScreeningLead, arsenic, cadmium & mercury — Pass
- Endotoxins (LAL)Bacterial endotoxin levels — Pass
- Sterility TestingNo microbial contamination — Pass
- Net Peptide ContentActual peptide mass per vial verified
Certificate of Analysis · Freedom DiagnosticPASS
- HPLC Purity
- ≥99%
- Identity
- Mass-spec confirmed
- Endotoxin (LAL)
- Pass
- Net content
- Verified
Compound Information
Full specification
| Chemical Name | 5-Amino-1-methylquinolinium |
| CAS Number | 4213-45-0 |
| Molecular Weight | 158.19 Da |
| Molecular Formula | C₁₀H₁₀N₂ |
| Target | Nicotinamide N-methyltransferase (NNMT) |
| Mechanism | Competitive NNMT enzyme inhibitor |
| Form | Lyophilized powder |
| Purity | ≥99% (HPLC verified) |
| Testing | Third-party HPLC, Mass Spec, Endotoxin |
| Storage | -20°C, protect from light |
| COA | Included with every order |
FAQ
Common questions about 5-Amino-1MQ
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using S-adenosyl methionine (SAM). NNMT is upregulated in adipose tissue in obesity, and its inhibition is studied as a potential strategy to modulate NAD+ metabolism and metabolic gene expression. It is used exclusively as a research tool compound.
NNMT metabolizes nicotinamide into 1-methylnicotinamide (MNA), diverting it away from the NAD+ salvage pathway. When NNMT is inhibited by compounds like 5-Amino-1MQ, more nicotinamide becomes available for conversion to NAD+ via NAMPT. Preclinical studies report approximately 1.6× increases in intracellular NAD+ following NNMT inhibition in adipocyte models.
In diet-induced obese (DIO) mouse models, NNMT inhibition led to approximately 35% reduction in white adipose tissue mass compared to controls. Importantly, food intake remained unchanged in treated animals, suggesting the compound's effects relate to energy expenditure pathways rather than appetite. These findings have not been replicated in human trials.
No. 5-Amino-1MQ is a mechanistically distinct research compound. Rather than directly supplying NAD+ precursors (like NMN or NR), it works by blocking an enzyme that consumes nicotinamide, theoretically preserving the substrate pool for endogenous NAD+ production. Its effects on overall NAD+ biology are still being characterized in preclinical research.
No. 5-Amino-1MQ has not been approved by the FDA or any regulatory agency for human use. No human clinical trials have been completed. It is sold exclusively as a research tool compound for in vitro laboratory use and is not intended for human consumption.
Store lyophilized 5-Amino-1MQ at -20°C and protect from light. For in vitro studies, it is typically dissolved in DMSO or aqueous buffer depending on the experimental protocol. Consult published literature for solubility parameters relevant to your research application.
Published Literature
Cited Sources
Peer-reviewed literature
Independent published research indexed from PubMed and peer-reviewed journals — not Eon Peptides claims.
[1]
PubMed
[2]Nicotinamide N-methyltransferase: More than a vitamin B3 clearance enzyme
View source PubMed
[3]Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity
View source PubMed
[4]Identification of novel NNMT inhibitors as potential anti-obesity agents
View source PubMed
Overexpression of nicotinamide N-methyltransferase in adipose tissue in obesity
View source For Research Use Only.
Not for human or veterinary use. For in-vitro laboratory research only. These statements have not been evaluated by the FDA; this product is not intended to diagnose, treat, cure, or prevent any disease. Sold exclusively to qualified researchers and institutions.