Peptide · Research Monograph · 43-amino acid fragment of Thymosin Beta-4 (Tβ4) active region

TB-500

Studied for faster healing, flexibility, and recovery

TB-500 is the active fragment of Thymosin Beta-4, researched for accelerating healing, flexibility, and recovery by helping cells migrate and rebuild tissue.

For laboratory research use only — not for human or animal use

Available in the Eon catalog — TB-500 from $85.00 Certificate of analysis (PDF)

Molecular data

Molecular formulaC212H350N56O78S
Molecular weight4963.44 Da
Sequence length43 residues
Physical formLyophilized Powder
Available sizes10mg

How it works

Actin Regulation

Cell Migration & Motility

TB-500 binds G-actin monomers and promotes actin polymerization, enabling cells to migrate to injury sites. This is the primary mechanism driving its tissue repair properties in preclinical models.

  • Sequesters G-actin to regulate polymerization
  • Promotes cell migration to injury sites
  • Enhances cytoskeletal remodeling
Cardiac

Cardiac Repair Signaling

In cardiac research, TB-500 activates integrin-linked kinase (ILK) and promotes cardiac progenitor cell migration. Phase II clinical trials have been conducted for cardiac repair applications.

  • Activates integrin-linked kinase (ILK)
  • Promotes cardiac progenitor cell migration
  • Phase II clinical trial data available
Anti-Inflammatory

Inflammation Modulation

TB-500 downregulates pro-inflammatory cytokines and modulates macrophage activity, shifting the immune response from inflammatory to regenerative in preclinical wound models.

  • Reduces pro-inflammatory cytokines
  • Modulates macrophage polarization
  • Supports anti-fibrotic tissue remodeling

What the research shows

Dermatology

Wound Healing

Accelerates dermal wound closure, reduces scarring, and promotes organized collagen deposition in preclinical wound models.

Malinda et al. 2007

Cardiovascular

Cardiac Repair

Promotes cardiac cell migration via ILK activation. Phase II clinical trials conducted for post-myocardial infarction repair.

Bock-Marquette et al. 2004

Ophthalmology

Corneal Healing

Promotes corneal epithelial wound healing and reduces inflammation in ocular surface damage models.

Sosne et al. 2018

Musculoskeletal

Soft Tissue Repair

Enhances muscle, tendon, and ligament repair through actin-mediated cell migration and reduced fibrotic scarring.

Goldstein et al. 2010

Specification

Active FragmentThymosin Beta-4 (Tβ4) active region
Amino Acids43-amino acid peptide
Molecular Weight4,963 Da
FormLyophilized powder (10mg)
Purity≥99% (HPLC verified)
TestingThird-party HPLC, Mass Spec, Endotoxin
Storage (lyophilized)-20°C for long-term stability
Storage (reconstituted)2–8°C, use within 14-21 days
SolubilityBacteriostatic water for reconstitution
COAIncluded with every order

Frequently asked questions

What is TB-500?

TB-500 is a synthetic peptide corresponding to the active region of Thymosin Beta-4 (Tβ4), a 43-amino acid protein naturally found in all nucleated cells. It plays a central role in cell migration, differentiation, and tissue repair through its interaction with the actin cytoskeleton.

How does TB-500 differ from Thymosin Beta-4?

TB-500 contains the active fragment of the full Thymosin Beta-4 protein. While Tβ4 is the complete 43-amino acid sequence, TB-500 focuses on the region responsible for actin binding and cell migration properties. Both share the same core mechanism of action.

Has TB-500 been in clinical trials?

Yes — Thymosin Beta-4 (RegeneRx Biopharmaceuticals) has been studied in Phase II clinical trials for cardiac repair after myocardial infarction, as well as corneal wound healing. These trials showed acceptable safety profiles. TB-500 as sold for research has not itself been in FDA-approved trials.

What are the primary research applications?

TB-500 is studied in preclinical models for wound healing, cardiac tissue repair, corneal healing, muscle/tendon recovery, and anti-inflammatory applications. Its primary mechanism — promoting cell migration via actin regulation — is relevant across multiple tissue types.

How should TB-500 be stored?

Store lyophilized TB-500 at -20°C for long-term stability. After reconstitution with bacteriostatic water, refrigerate at 2-8°C and use within 14-21 days. Avoid repeated freeze-thaw cycles.

Is TB-500 FDA approved?

No. TB-500 is not FDA approved for any indication. It is available exclusively for in vitro laboratory research. While the parent molecule (Thymosin Beta-4) has been in clinical trials, TB-500 itself has not received regulatory approval.

Literature

  • PubMed Thymosin beta-4 promotes dermal healing Malinda KM et al. — 2007
  • PubMed Thymosin beta 4 activates integrin-linked kinase and promotes cardiac cell migration Bock-Marquette I et al. — 2004
  • PubMed Thymosin beta-4 and the eye: Current concepts and therapeutic potential Sosne G et al. — 2018
  • PubMed The role of thymosin beta-4 in tissue repair and remodeling Goldstein AL et al. — 2010

For laboratory research use only. Not a drug, supplement, or medical product; not for human or animal use. All findings referenced are from published preclinical/laboratory research.